简介

The focus of my research is the response of cells and tissues to mechanical loading. In the cardiovascular system, I am interested in mechanotransduction mechanisms responsible for the endothelial cell response to flow. The areas of expertise I have brought to bear on this problem are: Atomic Force Microscopy (AFM) of living cells in culture to measure the three-dimensional surface topography Computational Fluid Dynamics (CFD) to calculate shear stress distributions by simulating flow over the surface geometries measured by AFM cell-culture models for applying shear stress to endothelial monolayers. I am also interested in the response of medial smooth muscle cells to the cyclic stretching that occurs in vivo due to the blood pulse. I have developed cell-culture models for applying a biaxial stretch to cultured cells while recording their responses by fluorescence microscopy techniques. In addition to the responses to physiological mechanical stimuli, I am also interested in the response of neural and vascular tissue to the extreme loading conditions associated with traumatic injury. The goals of this work are to establish cellular injury criteria that can be used in the development of protective equipment and to provide an injury model in which the mechanical insult is precisely controlled, the cellular response is measured, and the ability of therapeutic agents to mitigate the injury can be evaluated. My teaching interests include cellular biomechanics, biological elasticity and viscoelasticity, cellular imaging, mechanics of cell adhesion and locomotion, and mathematical modeling of cellular processes.