Heterotrimeric (αβγ) G proteins control major signal transduction pathways in eukaryotes. Their importance is underscored by the fact that more than 30% of pharmaceutical drugs target G protein signaling pathways. My laboratory discovered heterotrimeric G proteins in filamentous fungi, using the model organism Neurospora crassa. My group has now characterized the myriad functions and physical interactions between three Gα, one Gβ and one Gγ proteins in Neurospora (Won et al. 2012, Eukaryot Cell: 11:1239-48). Since our original discovery, Gα proteins have been implicated in pathogenesis in numerous plant and animal fungal pathogens (reviewed in Li et al. 2007, Ann. Rev. Microbiol. 61:423-52). Therefore, our work is relevant to development of new infection control practices and identification of targets for chemical agents to combat fungal diseases in plants and animals.