简介

Transcriptional control in development During embryogenesis, a cluster of apparently undifferentiated cells is transformed into an ordered array of differentiated tissues. Using Drosophila as a model system, my research group combines biochemical and genetic approaches to study the molecular basis of this amazing transformation. Essentially all the regulatory circuits we study are conserved throughout the animal kingdom. Therefore, our studies have important implications for human health and development. The following two major projects are currently underway in the lab. 1) Spatial and temporal regulation of transcription in development. We have been extensively examining mechanisms of activation and repression by the Dorsal morphogen, a transcription factor that determines the dorsal/ventral axis during early development. This factor is the Drosophila homolog of the vertebrate regulatory protein NF-kB. Like Dorsal, NF-kB is involved in both the determination of embryonic polarity and in the innate immune response. Furthermore, both Dorsal and NF-kB are regulated by homologous signal transduction cascades that control transcription factor activity by regulating nuclear import. 2) Role of Sumo-conjugation in development. Sumo is a recently discovered member of the ubiquitin family that is conserved throughout all eukaryotes. This polypeptide is a substrate for a protein conjugation system, in which Sumo becomes covalently attached to numerous target proteins modifying their behavior in various ways. We are attempting to learn about the roles of Sumo in cell biology and development. Our analysis has revealed possible roles for this process in regulated nuclear import, embryonic pattern formation, the immune response, and the stress response. Additional information about my research program is available at the following address: Click for more information on Courey Lab.